Mice with Anxa-2 gene floxed. Annexin 2 is a calcium-dependent phospholipid-binding protein involved in exocytosis, cell motility, cytoskeleton organization, and cell matrix interaction. Linked to lupus nephritis. This mouse line is currently under development.
Mice with epithelial membrane protein 2 gene floxed. Conditionally targeted this gene encodes an endometrial protein necessary for blastocyst implantation, but also controls delivery of certain classes of proteins to the cell surface, including various integrin isoforms. Mutations in EMP2 have been identified in patients with nephrotic syndrome. This mouse line is currently under development.
Mice with FMS-like tyrosine kinase 3 gene floxed. The gene is identified in familial nephrotic syndrome. This mouse line is currently under development.
Serine/threonine protein kinase that regulates cell growth, proliferation, motility, survival, protein synthesis, and transcription. MTOR is the target of rapamycin and activation of MTOR linked to diabetic kidney disease.
Mice with transcription factor 21 gene floxed. It is highly expressed in the kidney and needed for metanephrogenesis and podocyte health. Cell-specific KOs provide models of CAKUT and nephrosis. Linked to fibrosis and fibroblast progenitors in heart and kidney and lung. Recently linked to tuberous sclerosis.
Mice with angiopoietin 1 gene floxed. It is a growth factor protein that promotes angiogenesis. It is the major agonist for the tyrosine kinase receptor, Tek, which is found primarily on endothelial cells. Important in diabetic kidney disease, AKI, sepsis and other renal-related diseases.
Mice with angiopoietin 2 gene floxed. It is a growth factor protein that promotes lymphangiogenesis. Increased Angpt2 levels are associated with adverse outcomes in AKI, sepsis etc.
This is a conditional model of Angpt4 gene, which is a ligand for Angpt/Tie Signaling pathway. This mouse line is currently under development.
Mice with protein tyrosine phosphatase, receptor type, B gene floxed. The phosphatase deactivates Tie2/Tek receptor. Target for vascular quiescence in multiple renal diseases including diabetic kidney disease.
Mice with tyrosine kinase with immunoglobulin-like and EGF-like domains 1 gene floxed. It is an angiopoietin receptor- cell surface protein- expressed exclusively in endothelial cells and absolutely required for glomerular endothelial development. This mouse line is currently under development.
Angiopoeitin receptor Tie2 is involved in formation of vasculature. A conditional knock out mouse model is under production.
Vascular endothelial growth factor A stimulates vasculogenesis and angiogenesis. A conditional mouse model that can be knocked out in a tissue specific manner is being generated. MTA pending. Permission to share this line may be needed. This mouse line is currently under development.
The receptor tyrosine kinase vascular endothelial growth factor receptor 3 (VEGFR-3, previously denoted fms-like tyrosine kinase-4 or Flt-4) is essential for the development of the blood and lymphatic vasculature. Inactivation of the VEGFR-3 gene in mouse embryos leads to a disturbed vascular development resulting in an irregular vessel pattern and a reduced cross-sectional area of large vessels. The embryos die at embryonic day 9.5 because of fluid accumulation in the pericardial cavity and cardiovascular failure. MTA pending. Permission to share this line may be needed. This mouse line is currently under development.
Cre and rtTA Lines
Cre is expressed under the podocyte specific Nephrin promoter. Nephrin is necessary for the proper functioning of the renal filtration barrier.
Cre is expressed under the metanephric-mesenchyme specific Tcf21 promoter. Deletes during kidney development in condensing mesenchyme and all derivatives (nephron epithelium).
Cre is expressed under the podocyte specific podocin promoter. Podocin protein is the component of the slit diaphragm of podocytes.
Tetracycline-controlled Transcriptional Activation regulated by Podocin promoter, which specifically expressed in podocytes.These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders. nducible podocyte-specific strain in podocytes.
Nephrin-CFP mice contain a CFP reporter gene that is driven by the nephrin promoter – high expression in podocytes.
In the model, Tcf21 null allele expressing b-galactosidase gene that tags nephron progenitors, stromal lineage and podocytes.
Transgenic mice exhibit brightly fluorescent in Angpt1 expressing cells as tdTomato is expressed under the direction of Angpt1 promoter. Myristoylation of myrTdT Myristoylation of myrTdT visualizes the localization of Angpt1 ligand. Under development. This mouse line is currently under development.
This transgene is random insertion of diphtheria toxin cDNA under control of beta-actin promoter. Permits cell-specific ‘killing’ of cells when crossed to appropriate Cre-driver strain – e.g. used as a model for podocytopenia and glomerulosclerosis.
Slc5a2 (Sweet Pee)
ENU mutagenized mouse model carrying a mutation on SGLT2 gene which is a renal tubular sodium-glucose transporter. Sweet pee mutants (in homozygous state) exhibit dramatic glucosuria. Heterozygotes resemble patients with familial glycosuria.
Mice with tetO-Cre/Vhlflox/flox transgenes can be induced for Hif2a gene excision with doxycycline induction when crossed with Tetracycline-controlled Transcriptional Activation regulated by tissue specific promoter. Hif2a involved in glomerular fibrosis and in renal cell carcinoma, as well as the integrity of the glomerular barrier.
Mice with R494X mutation in angiopoietin 1 gene. Mutation predicted to be hypomorphic and identified in cases with vascular defects.
This mouse lacks introns 13 and 14 which possess regulatory elements to create various soluble isoforms of the Flt1 gene. It has been generated to study the effect of these regulatory elements in preeclampsia and TMAs. This mouse line is currently under development.