Northwestern University Feinberg School of Medicine
nephroHUB

Available Mouse Lines

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Conditional Lines

Anxa2 flox

Anxa2tm1a(EUCOMM)Seq

Mice with Anxa-2 gene floxed. Annexin 2 is a calcium-dependent phospholipid-binding protein involved in exocytosis, cell motility, cytoskeleton organization, and cell matrix interaction. Linked to lupus nephritis. This mouse line is currently under development.

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Emp2 flox

Emp2tm1a(KOMP)Seq

Mice with epithelial membrane protein 2 gene floxed. Conditionally targeted this gene encodes an endometrial protein necessary for blastocyst implantation, but also controls delivery of certain classes of proteins to the cell surface, including various integrin isoforms. Mutations in EMP2 have been identified in patients with nephrotic syndrome. This mouse line is currently under development.

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Flt3 flox

Flt3tm1.1Seq

Mice with FMS-like tyrosine kinase 3 gene floxed. The gene is identified in familial nephrotic syndrome. This mouse line is currently under development.

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mTOR flox

mTORtm1.1Seq

Serine/threonine protein kinase that regulates cell growth, proliferation, motility, survival, protein synthesis, and transcription. MTOR is the target of rapamycin and activation of MTOR linked to diabetic kidney disease.

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Tcf21 flox

Tcf21tm2.1Seq

Mice with transcription factor 21 gene floxed. It is highly expressed in the kidney and needed for metanephrogenesis and podocyte health. Cell-specific KOs provide models of CAKUT and nephrosis. Linked to fibrosis and fibroblast progenitors in heart and kidney and lung. Recently linked to tuberous sclerosis.

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Angpt1 flox

Angpt1tm1.1Seq

Mice with angiopoietin 1 gene floxed. It is a growth factor protein that promotes angiogenesis. It is the major agonist for the tyrosine kinase receptor, Tek, which is found primarily on endothelial cells. Important in diabetic kidney disease, AKI, sepsis and other renal-related diseases.

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Angpt2 flox

Angpt2tm92002(LIL2_Pgk_P)Seq

Mice with angiopoietin 2 gene floxed. It is a growth factor protein that promotes lymphangiogenesis. Increased Angpt2 levels are associated with adverse outcomes in AKI, sepsis etc.

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Angpt4 flox

Angpt4tm1a(KOMP)Seq

This is a conditional model of Angpt4 gene, which is a ligand for Angpt/Tie Signaling pathway. This mouse line is currently under development.

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Flk1 flox

Vegfr-2tm1.1

Receptor Tyrosine Kinase 2 gene for VEGF is floxed. Master regulator of angiogenesis. Loss of Flk1 + cells linked to progression from AKI to CKD.

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Flt1 flox

Vegfr-1tm1.1

Receptor Tyrosine Kinase 1 for VEGF is floxed. Gene linked to preeclampsia and other kidney diseases.

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Nrp1 flox

Nrp1tm2

Neuropilin is a transmembrane co-receptor for VEGF and Semaphorins. Highly expressed in glomerular microvasculature.

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Prox1 flox

Prox1tm1

When bred to Cre recombinase expressing mice, the resulting offspring carry floxed alleles of the prospero homeobox 1 gene, critically important for lymphangiogenesis including renal lymphatics.

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Ptprb flox

Ptprbtm2a(EUCOMM)Seq

Mice with protein tyrosine phosphatase, receptor type, B gene floxed. The phosphatase deactivates Tie2/Tek receptor. Target for vascular quiescence in multiple renal diseases including diabetic kidney disease.

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Tie1 flox

Tie1tm1a(KOMP)Seq

Mice with tyrosine kinase with immunoglobulin-like and EGF-like domains 1 gene floxed. It is an angiopoietin receptor- cell surface protein- expressed exclusively in endothelial cells and absolutely required for glomerular endothelial development. This mouse line is currently under development.

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Tie2 Floxed

Angiopoeitin receptor Tie2 is involved in formation of vasculature. A conditional knock out mouse model is under production.

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Vegfa flox

Vascular endothelial growth factor A stimulates vasculogenesis and angiogenesis. A conditional mouse model that can be knocked out in a tissue specific manner is being generated. MTA pending. Permission to share this line may be needed. This mouse line is currently under development.

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Cre and rtTA Lines

Foxd1Cre

Cre expression is regulated by FoxD1 promoter. FoxD1 is a transcription factor required for morphogenesis of the kidney.These mice are useful for studying therapeutic strategies directly targeting pericyte differentiation in vivo and may productively impact fibrotic kidney disease. Deletion affects stromal lineage in kidney.

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Nephrin Cre

Nphs1tm1(cre)Seq

Cre is expressed under the podocyte specific Nephrin promoter. Nephrin is necessary for the proper functioning of the renal filtration barrier.

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Pax8-rtTA;tetOCre

rtTA sequence under the control of the murine Pax8 promoter.These Pax8-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in renal tubular epithelial cells, and may be useful in studying renal disorders such as fibrosis or polycystic kidney disease, renal cancer, and Tuberous Sclerosis. Coumpound line not available at JAX.

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PDGFRb-Cre

Cre recombinase gene under the control of Pdgfrb promoter. Expressed in mesangial cells of kidney.

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Pdgfrb-CreERT2

Tamoxifen inducible cre recombinase gene under the control of Pdgfrb promoter. Inducible deletion in mesangial cells.

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Pod1Cre

Tcf21tm1(cre)Seq

Cre is expressed under the metanephric-mesenchyme specific Tcf21 promoter. Deletes during kidney development in condensing mesenchyme and all derivatives (nephron epithelium).

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Podocin Cre

Tg(Nphs2-cre)1Seq

Cre is expressed under the podocyte specific podocin promoter. Podocin protein is the component of the slit diaphragm of podocytes.

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Podocin-rtTA; tetOCre

Tetracycline-controlled Transcriptional Activation regulated by Podocin promoter, which specifically expressed in podocytes.These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders. nducible podocyte-specific strain in podocytes.

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Podoplanin Cre

Tg(Pdpn-CreGFP)G0

Pdpn is a transmembrane mucin-type O-glycoprotein that starts to express on the surface of lymphatic Endothelial Cells (LECs) and podocytes. The LECs begin expressing Pdpn as it develops into mature LECs. The Cre recombination results in deletion of gene in mature LECs and podocytes.

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Six2 Cre

Homeobox protein involved in the regulation of patterns of anatomical development. Highly expressed in kidney progenitors.

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Wnt4Cre, GFP

Wnt4tm2(EGFP/cre)Svo

Cre is expressed under the Wnt4 promoter. It provides a useful tool for conditional mutagenesis and provides the first time-lapse-based map of the fate of nephron precursor cells.

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CDH5 CreERT2 (RA)

In this transgenic line, the Cre-ERT2 expression cassette was placed under the regulatory control of the mouse vascular endothelial cadherin 5 promoter. It is a valuable tool to study the function of genes involved in vascular development, homeostasis, and in complex processes involving neoangiogenesis, such as tumor growth and in the kidney.

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Emcn-CreERT2

This model has a tamoxifen inducible Cre-mediated recombination system in Endomucin expressing (venous endothelial) cells. This mouse line is currently under development.

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Emcn-rtTA

rtTA sequence under the control of the murine Endomucin promoter.These Emcn-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in venous endothelial cells of mice. This mouse line is currently under development.

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Prox1CreERT2

This model has a tamoxifen inducible Cre-mediated recombination system in Prox1 expressing cells. Lymphatic specific deletion.

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Prox1GFPCre

Prox1tm5(GFP/cre)1

GFPCre is inserted into Prox1 genomic locus, resulting in haploinsufficient mice and deletion in lymphatic endothelium. Generated by O.Giermo Lab.

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Ptprb-NLS-Cre

This model has a Cre-mediated recombination system in Ptprb expressing cells. This mouse line is currently under development.

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Ptprb-NLS-CreERT2

This model has a tamoxifen inducible Cre-mediated recombination system in Ptprb expressing cells. This mouse line is currently under development.

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Reporter Lines

Nephrin CFP

Nephrin-CFP mice contain a CFP reporter gene that is driven by the nephrin promoter – high expression in podocytes.

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Pod1 LacZ

Tcf21lacZ

In the model, Tcf21 null allele expressing b-galactosidase gene that tags nephron progenitors, stromal lineage and podocytes.

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Angpt1-myrTdT

Tg(Angpt1-MyrTdT)SQ

Transgenic mice exhibit brightly fluorescent in Angpt1 expressing cells as tdTomato is expressed under the direction of Angpt1 promoter. Myristoylation of myrTdT Myristoylation of myrTdT visualizes the localization of Angpt1 ligand. Under development. This mouse line is currently under development.

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Flk1eGFP

Flk1-EGFP

Flk1 EGFP knock-in.

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Flt1LacZ

Flt-1+/−

Targeted allele of Flt-1 carries a lacZ knock-in sequence. Mice heterozygous for the Flt-1 gene.

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Prox1 GFP

Tg(Prox1-EGFP)

Recapitulates the expression pattern of the Prox1 gene using a GFP reporter. This line is used to conveniently visualize detailed structure and morphology of lymphatic vessels and networks throughout development.

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Prox1 LacZ/+

Prox1-null

Mice heterozygous for the Prox1 gene with lacZ expression in lymphatic endothelial cells.

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Tie2-myrTdT

Knock in mice exhibit brightly fluorescent in Tie2 expressing cells as tdTomato is expressed under the direction of Tie2 promoter. Myristoylation of myrTdT visualizes the localization of Tie2 receptor. This mouse line is currently under development. This mouse line is currently under development.

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Vegfr3 LacZ/+

Vegfr3-null

Mice heterozygous for the FMS-like tyrosine kinase 4 gene. Expressed highly in peritubular capillaries in the kidney.

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Vegfr3YFP

Vegfr3 :YFP

A transgenic reporter mouse for neural stem cells and lymphatic vessels. YFP is expressed under Vegfr3 promoter. Vegfr3 protein is involved in lymphangiogenesis and maintenance of the lymphatic, glomerular endothelium as well as peritubular kidney capillaries.

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VEPTP LacZ

VEPTP-null

Promoter-less IRES-lacZ-pgk-neo cassette is under the control of the endogenous VE-PTP promoter. Strongly expressed in glomerular endothelial cells. MTA pending. Permission to share this line may be needed.

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Other Lines

PTH

This transgene is random insertion of diphtheria toxin cDNA under control of beta-actin promoter. Permits cell-specific ‘killing’ of cells when crossed to appropriate Cre-driver strain – e.g. used as a model for podocytopenia and glomerulosclerosis.

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Samd9L

This mouse generated with ENU mutagenesis. It carries S619T mutation on sterile alpha motif domain containing 9-like gene. The phenotype resembles the autosomal dominant polycystic kidney disease.

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Slc5a2 (Sweet Pee)

ENU mutagenized mouse model carrying a mutation on SGLT2 gene which is a renal tubular sodium-glucose transporter. Sweet pee mutants (in homozygous state) exhibit dramatic glucosuria. Heterozygotes resemble patients with familial glycosuria.

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TetoHIF2a

Mice with tetO-Cre/Vhlflox/flox transgenes can be induced for Hif2a gene excision with doxycycline induction when crossed with Tetracycline-controlled Transcriptional Activation regulated by tissue specific promoter. Hif2a involved in glomerular fibrosis and in renal cell carcinoma, as well as the integrity of the glomerular barrier.

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Angpt1-R494X

Mice with R494X mutation in angiopoietin 1 gene. Mutation predicted to be hypomorphic and identified in cases with vascular defects.

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CD2AP knockout

B6.129X1-Cd2aptm1Shaw/J

The sequence encoding the first Src homology 3 domain is replaced with neomycin casette. Homozygote present severe proteinuria and die at neonate. Heterozygote presents proteinuria 8-10 months old and variable degree of glomerulosclerosis.

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Flt1-C159S

Mice with C159S mutation in Vegf binding domain of Flt1 gene. It has been generated to study the effect of these regulatory elements in preeclampsia and TMAs. This mouse line is currently under development.

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Flt1Fused

This mouse lacks introns 13 and 14 which possess regulatory elements to create various soluble isoforms of the Flt1 gene. It has been generated to study the effect of these regulatory elements in preeclampsia and TMAs. This mouse line is currently under development.

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FltTK-/-

The tyrosine kinase (TK) domain of VEGFR1 deleted in this model.

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FltTMTK-/+

The transmembrane (TM) domain and the tyrosine kinase (TK) domains of VEGFR1 deleted in this model.

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Vegfc +/-

Mice heterozygous for the vascular endothelial growth factor C gene.

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