Available Mouse Lines
To request a mouse line, click the Request button associate with the line to access the OKRA Resource Consultation Form. Select NU-USC as the resource center, then in the “In a few short sentences, please tell us your project needs or list the resource(s) you intend to request from the selected center(s)” field, enter the name of the mouse line you are requesting along with any additional information that may help us prepare for your consultation.
Conditional Lines
Anxa2 flox
Anxa2tm1a(EUCOMM)SeqMice with Anxa-2 gene floxed. Annexin 2 is a calcium-dependent phospholipid-binding protein involved in exocytosis, cell motility, cytoskeleton organization, and cell matrix interaction. Linked to lupus nephritis. This mouse line is currently under development.
Emp2 flox
Emp2tm1a(KOMP)SeqMice with epithelial membrane protein 2 gene floxed. Conditionally targeted this gene encodes an endometrial protein necessary for blastocyst implantation, but also controls delivery of certain classes of proteins to the cell surface, including various integrin isoforms. Mutations in EMP2 have been identified in patients with nephrotic syndrome. This mouse line is currently under development.
Flt3 flox
Flt3tm1.1SeqMice with FMS-like tyrosine kinase 3 gene floxed. The gene is identified in familial nephrotic syndrome. This mouse line is currently under development.
mTOR flox
mTORtm1.1SeqSerine/threonine protein kinase that regulates cell growth, proliferation, motility, survival, protein synthesis, and transcription. MTOR is the target of rapamycin and activation of MTOR linked to diabetic kidney disease.
Tcf21 flox
Tcf21tm2.1SeqMice with transcription factor 21 gene floxed. It is highly expressed in the kidney and needed for metanephrogenesis and podocyte health. Cell-specific KOs provide models of CAKUT and nephrosis. Linked to fibrosis and fibroblast progenitors in heart and kidney and lung. Recently linked to tuberous sclerosis.
Nck1 mt/wt Nck2 fl/fl
The Nck family of adaptor proteins (Nck1 and Nck2) serves as a crucial signaling bridge. They link the phosphorylated slit diaphragm protein nephrin to the underlying actin cytoskeleton.
Angpt1 flox
Angpt1tm1.1SeqMice with angiopoietin 1 gene floxed. It is a growth factor protein that promotes angiogenesis. It is the major agonist for the tyrosine kinase receptor, Tek, which is found primarily on endothelial cells. Important in diabetic kidney disease, AKI, sepsis and other renal-related diseases.
Angpt2 flox
Angpt2tm92002(LIL2_Pgk_P)SeqMice with angiopoietin 2 gene floxed. It is a growth factor protein that promotes lymphangiogenesis. Increased Angpt2 levels are associated with adverse outcomes in AKI, sepsis etc.
Angpt4 flox
Angpt4tm1a(KOMP)SeqThis is a conditional model of Angpt4 gene, which is a ligand for Angpt/Tie Signaling pathway. This mouse line is currently under development.
Ptprb flox
Ptprbtm2a(EUCOMM)SeqMice with protein tyrosine phosphatase, receptor type, B gene floxed. The phosphatase deactivates Tie2/Tek receptor. Target for vascular quiescence in multiple renal diseases including diabetic kidney disease.
Tie1 flox
Tie1tm1a(KOMP)SeqMice with tyrosine kinase with immunoglobulin-like and EGF-like domains 1 gene floxed. It is an angiopoietin receptor- cell surface protein- expressed exclusively in endothelial cells and absolutely required for glomerular endothelial development. This mouse line is currently under development.
Vegfa flox
Vascular endothelial growth factor A stimulates vasculogenesis and angiogenesis. A conditional mouse model that can be knocked out in a tissue specific manner is being generated. MTA pending. Permission to share this line may be needed. This mouse line is currently under development.
Vegfr3flox
Flt4tm1a(KOMP)WtsiThe receptor tyrosine kinase vascular endothelial growth factor receptor 3 (VEGFR-3, previously denoted fms-like tyrosine kinase-4 or Flt-4) is essential for the development of the blood and lymphatic vasculature. Inactivation of the VEGFR-3 gene in mouse embryos leads to a disturbed vascular development resulting in an irregular vessel pattern and a reduced cross-sectional area of large vessels. The embryos die at embryonic day 9.5 because of fluid accumulation in the pericardial cavity and cardiovascular failure. MTA pending. Permission to share this line may be needed. This mouse line is currently under development.
VEPTP Flox
Used to study vascular stability, renal injury, and endothelial signaling.
VEGF FX
Mouse line used to study the kidney's filtration barrier is maintained.
VEGF-C flox
Used to study lymphangiogenesis, vascular development, and tissue-specific functions of vascular endothelial growth factor C (VEGF-C) by allowing researchers to delete the gene in targeted cell types when combined with specific Cre-driver lines.
Tie2 lcoin(Inverted)
Line used to study the Angiopoietin-Tie2 (Tek) signaling pathway in vascular and lymphatic development.
sFlt1 Flox
A conditional mouse model used to study autocrine functions of sFLT1 in tissue homeostasis from its systemic, damaging endocrine effects (such as the endothelial injury, hypertension, and preeclampsia-like phenotypes seen when sFLT1 is flooded into the bloodstream).
Tie2 flox
Angiopoeitin receptor Tie2 is involved in formation of vasculature. A conditional knock out mouse model is under production.
Cre and rtTA Lines
Nephrin Cre
Nphs1tm1(cre)SeqCre is expressed under the podocyte specific Nephrin promoter. Nephrin is necessary for the proper functioning of the renal filtration barrier.
Pod1Cre
Tcf21tm1(cre)SeqCre is expressed under the metanephric-mesenchyme specific Tcf21 promoter. Deletes during kidney development in condensing mesenchyme and all derivatives (nephron epithelium).
Podocin Cre
Tg(Nphs2-cre)1SeqCre is expressed under the podocyte specific podocin promoter. Podocin protein is the component of the slit diaphragm of podocytes.
Podocin-rtTA; tetOCre
Tetracycline-controlled Transcriptional Activation regulated by Podocin promoter, which specifically expressed in podocytes.These podocin-rtTA mice provide a Tet-On tool that allows dox-inducible expression of genes in podocytes, and may be useful in studying the role of podocyte nephrobiology in renal disorders. nducible podocyte-specific strain in podocytes.
Nephrin Cre-2
Neph-Cre line used in the study of paracrine signaling pathways within the renal filtration system.
Podocin Cre-2
The NPHS2-Cre (podocin-Cre) mouse model is a podocyte-specific Cre recombinase driver line. Widely used to cross with floxed target mice (flox/flox) to generate conditional knockout or gain-of-function models restricted to podocytes (e.g., studying diabetic nephropathy, focal segmental glomerulosclerosis, or glomerular basement membrane dynamics).
A1;A2;A4(NO);RosartTA; TetOcre
These three proteins serve as key ligands for the TEK (Tie2) receptor tyrosine kinase and play important roles in vascular stabilization, angiogenesis, and vascular permeability. This specialized triple-knockout system is primarily used to investigate vascular ligand redundancy and function, ocular disease, and tumor microenvironments. The knockout can be induced using the Rosa-rtTA/TetO-Cre system.
A1;A2;RosartTA;TetOcre
This inducible genetic system is widely used in vascular biology, oncology, and ophthalmology research to investigate the Angiopoietin/TEK (Tie2) signaling pathway.
Reporter Lines
Nephrin CFP
Nephrin-CFP mice contain a CFP reporter gene that is driven by the nephrin promoter – high expression in podocytes.
Pod1 LacZ
Tcf21lacZIn the model, Tcf21 null allele expressing b-galactosidase gene that tags nephron progenitors, stromal lineage and podocytes.
Angpt1-myrTdT
Tg(Angpt1-MyrTdT)SQTransgenic mice exhibit brightly fluorescent in Angpt1 expressing cells as tdTomato is expressed under the direction of Angpt1 promoter. Myristoylation of myrTdT Myristoylation of myrTdT visualizes the localization of Angpt1 ligand. Under development. This mouse line is currently under development.
Other Lines
PTH
This transgene is random insertion of diphtheria toxin cDNA under control of beta-actin promoter. Permits cell-specific ‘killing’ of cells when crossed to appropriate Cre-driver strain – e.g. used as a model for podocytopenia and glomerulosclerosis.
Slc5a2 (Sweet Pee)
ENU mutagenized mouse model carrying a mutation on SGLT2 gene which is a renal tubular sodium-glucose transporter. Sweet pee mutants (in homozygous state) exhibit dramatic glucosuria. Heterozygotes resemble patients with familial glycosuria.
TetoHIF2a
Mice with tetO-Cre/Vhlflox/flox transgenes can be induced for Hif2a gene excision with doxycycline induction when crossed with Tetracycline-controlled Transcriptional Activation regulated by tissue specific promoter. Hif2a involved in glomerular fibrosis and in renal cell carcinoma, as well as the integrity of the glomerular barrier.
Angpt1-R494X
Mice with R494X mutation in angiopoietin 1 gene. Mutation predicted to be hypomorphic and identified in cases with vascular defects.
Flt1Fused
This mouse lacks introns 13 and 14 which possess regulatory elements to create various soluble isoforms of the Flt1 gene. It has been generated to study the effect of these regulatory elements in preeclampsia and TMAs. This mouse line is currently under development.
Nrp1 flox
floxed Nrp1 mouse line crossed with tissue-specific Cre-recombinase drivers, used to study critical mechanisms governing glomerular function and drug toxicities.
Samd9L
The Samd9L mouse model is a genetic tool used to study human bone marrow failure and blood disorders caused by SAMD9 and SAMD9L gene mutation.